Transient protein expression, is also known as transient gene expression. The proteins are expressed for a short duration. Majorly transient protein expressions are done with cultured animal cells. The technique is used in plant cells as well, however the transfer of proteins into these cells requires different methods than those with animal cells.
Stable transfer of cells and microorganisms is the key element for manufacturing of therapeutic proteins. Stably transfected mammalian cells in culture medium expand and further express their protein producing traits to their offspring’s. In transient protein expression new genes enter the host body carrying some protein making capability without becoming the component of organism’s genome. Various existing chemical and physical methods are used for achieving transient protein expression, these methods includes electroporation, gene guns and many others. These techniques impose limitations on per cell and volumetric productivity of transient protein production.
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Transient protein expression is a speedy process when compared to stable integration. The stable transfection takes months and weeks while transient protein expression is accomplished in days, providing reliable and rapid access to small quantities of proteins for experimentation as well as characterization. Chemical based transient protein expression methods are used majorly due to low cost of transfection reagents and ease of use. Lipofection is one of the chemical based transient protein expression method which uses liposomes. The physical based transient protein expression method includes electroporation and uses high voltage electric shocks which induces DNA into the cells.
Factors Transient Protein Expression Market
The Transient Protein Expression market is driving with the increase in development of genetic engineering which has further opened many avenues and possibilities of expression and isolation of proteins in large scale. The various transient protein expression systems are yeast, bacteria, insect and mammalian systems. Considerable advances in technology and research have enabled transient protein expressions and isolation of recombinant proteins in large scale. However the success of transient protein expression depends on reproducibility, transfection efficiency which requires efficient researchers and reliable reagents to enhance the cell culture conditions. Moreover the market for transient protein expression is expected to increase due to its relatively inexpensive cost when compared to stable transfection also the timeline of expression is rapid. The transient protein expression is preferred more for small scale protein expression due to its rapid results.
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Moreover one of the major factor driving the improvement of the market includes increasing public-private funding for life science, research and presently commercially available very high-throughput devices for transient protein expressions. Healthcare facilities such as hospitals have changed their focus to a better value based system owing to improvement in healthcare. These factors stimulate the R&D and medical departments to overcome the deficiencies for better alternatives in Transient Protein Expressions.
Some of the major players in the Transient Protein Expression Market are Merck KGaA, Lonza, Thermo Fisher Scientific, PhyNexus, Inc. Molecular Devices, LLC, Sartorius AG, Promega Corporation among others. Moreover, the manufacturers of Transient Protein Expressions are mainly focusing on strengthening and increasing the core competencies of their product portfolio.
Transient Protein Expression Market: Segmentation
Tentatively, Transient Protein Expression market has been segmented on the basis of Product type, Applications, End User and Geography.
Based on Product type, the Transient Protein Expression market is segmented as:
- Automated Transient Protein Expression Systems
- Manual Transient Protein Expression Systems
- Western Blotting
- Fluorescence microscopy
Based on Applications, the Transient Protein Expression market is segmented as:
- Chemical based transient protein expression
- Calcium phosphate exposure
- Cationic lipofection
- Physical based transient protein expression
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Based on End User, the Transient Protein Expression Market is segmented as:
- Specialty clinics
- Academic and Research Institutes
Based on region, Transient Protein Expression Market is segmented as:
- North America
- Latin America
- Asia Pacific Excluding Japan
- Middle East & Africa
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