Hi guys!

I'm doing electroporation on yeast petite strains (cannot utilize fermentable carbon source) and every time I only get few colonies. Originally, I thought because it's petite that's why I have very low transformation efficiency. But I tried doing it in the wild type and I still get few colonies. So now I think my protocol is the problem. Do you have any suggestions on how to improve my electroporation efficiency? I'm just doing the normal LiAc protocol.

Thank you very much!



Source link