The study of Bacillus sphaericus Ts-1 protoplast-plasmid transformation of electroporation
Wang Y.; Jiang B.; Jiao R.
1991-04-01
Source Publication Wei sheng wu xue bao = Acta microbiologica Sinica
ISSN 00016209
Volume 31Issue:2Pages:94-99
Abstract This report gave the best conditions of Bacillus sphaericus Ts-1 protoplast-plasmid pHV33 electroporation. The highest transformation frequency and transformation efficiency induced by three pulse of 21 KV/cm and 10 microseconds duration applied at an interval of one sec., was 2.44 x 10(2) transformants/micrograms DNA and 3.16 x 10(-6) respectively. The saturated concentration of DNA absorbed by the protoplast was 5 micrograms DNA/10(9) cells/ml. By means of this method, pJB417, a recombinant mosquito larvicide clone, was introduced into B. subtilis 168M and B. sphaericus Ts-1. The transformants of B. subtilis 168M with biocide activity were obtained, but the toxicity of B. sphaericus Ts-1 was not increased.
URL View the original
Language 英語
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Document Type Journal article
Collection University of Macau
Affiliation Nankai University
Recommended Citation
GB/T 7714

Wang Y.,Jiang B.,Jiao R.. The study of Bacillus sphaericus Ts-1 protoplast-plasmid transformation of electroporation[J]. Wei sheng wu xue bao = Acta microbiologica Sinica,1991,31(2):94-99.
APA
Wang Y.,Jiang B.,&Jiao R..(1991).The study of Bacillus sphaericus Ts-1 protoplast-plasmid transformation of electroporation.Wei sheng wu xue bao = Acta microbiologica Sinica,31(2),94-99.
MLA
Wang Y.,et al."The study of Bacillus sphaericus Ts-1 protoplast-plasmid transformation of electroporation".Wei sheng wu xue bao = Acta microbiologica Sinica 31.2(1991):94-99.


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