Available online 27 July 2021, 107912



Reversible electroporation followed by resting enhanced Thai basil survivability.

PEF with resting may provide a protective effect to reduce damage caused by drying.

The preservation of cell vitality increased the rehydration capacity of leaves .


Horticultural crops have a low tolerance to dehydration. In this paper, we show that the reversible electroporation (200 monopolar, rectangular pulses of 50 µs pulse duration, 760 µs between pulses and nominal field strength of 650 V/cm) of Thai basil leaves followed by 24 h resting before hot air drying at 40 °C enhanced the survivability of the tissues at certain levels of dehydration (moisture ratio = 0.2 and 0.1). However, this increased survival was rather limited. Through measurements of metabolic heat production during resting, rehydration kinetics, respiration and photosynthesis of the rehydrated leaves, we show that resting after the application of a reversible pulse-electric field (PEF) may allow a phase of hardening that has a protective effect on the cells, thus decreasing damage during the subsequent drying phase. Increased preservation of cell vitality would be associated with a more turgid and fresh-like rehydrated product, as cells would have the capacity to retain the rehydration water.


Ion leakage

Pulsed electric field


Stress responses

Viability preservation

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© 2021 Published by Elsevier B.V.

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