Highlights

3D nanoroded device is developed to achieve the synchronized intracellular and extracellular recording of cardiomyocytes.

Intracellular and extracellular signals are synchronously acquired by the low-voltage 3D nanoroded electroporation.

Intracellular and extracellular signals are effectively separated by customized MATLAB signal processing program.

The correlation of intracellular and extracellular signals is verified in both experimental and simulated way.

Abstract

Electrophysiological study is an essential and significant strategy to explore the biological mechanism of electrogenic cells. Current advanced nanodevices can achieve the high-fidelity intracellular electrophysiological recordings, and most of detection systems record the extracellular and intracellular action potentials (EAPs and IAPs) in an asynchronous or isolated manner, so it is demanded to develop the platform to reveal correlation between EAP and IAP recording. Here, we establish a utility strategy to achieve synchronized intracellular and extracellular recording of neonatal rat cardiomyocytes by low-voltage three-dimensional (3D) nanoroded electroporation. By integrating the advantages of nanodevice and microdevice, 3D nanoroded microdevice is developed to achieve the high-throughput large-scale synchronous intracellular and extracellular electrophysiological study. By applying low-voltage electroporation, intracellular and extracellular signals can be synchronously acquired from intracellular access and extracellular coupling, respectively. Recorded synchronized signals contain both typical EAPs and IAPs, which have good synchronicity in spatiotemporal dimensions at each recording site. Moreover, correlation between both signals is further bridged in experimental and simulated way. This intracellular electrophysiological platform presents unique advantages over the conventional system to achieve the synchronized intracellular and extracellular electrophysiological study at membrane voltage level.

Keywords

Intracellular and extracellular electrophysiology

Synchronized recording

Nanoroded multielectrode array

Electroporation

Cardiomyocyte

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