Intravital imaging of mouse embryos

Qiang Huang, Malkiel A. Cohen, Fernando C. Alsina, Garth Devlin, Aliesha Garrett,
Jennifer McKey, Patrick Havlik, Nikolai Rakhilin, Ergang Wang, Kun Xiang, Parker
Mathews, Lihua Wang, Cheryl Bock, Victor Ruthig, Yi Wang, Marcos Negrete, Chi Wut
Wong, Preetish K. L. Murthy, Shupei Zhang, Andrea R. Daniel, David G. Kirsch, Yubin
Kang, Blanche Capel, Aravind Asokan, Debra L. Silver, Rudolf Jaenisch, Xiling Shen

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S8
  • References

Images, Video, and Other Media

Movie S1
Embryonic heartbeat, indicating that the embryos were still alive.
Movie S2
Pups were delivered normally and survived birth.
Movie S3
The dam fed the pups normally.
Movie S4
Imaging a whole Wnt1-cre:tdTomato mouse embryo from E11.5 to E11.75 using a stereoscopic
microscope.
Movie S5
Synaptic transmission between neurons, imaged using the calcium indicator GCaMP.
Movie S6
Diffusion of fluorescein into an embryo at E15.5 following injection into the dam
orbital vein.
Movie S7
Movement of cells in an embryo at E14.5, 24 h after in utero electroporation to introduce pCAG-mCherry.
Movie S8
Cell migration in an embryo at E15.5, 24 h after in utero electroporation to introduce pCAG-eGFP.
Movie S9
Continuous imaging of a whole chimeric embryo, injected with eGFP-labeled mESCs in
blastocyst stage, for 12 h, from E11.5 to E12.0.



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