Intravital imaging of mouse embryos
Qiang Huang, Malkiel A. Cohen, Fernando C. Alsina, Garth Devlin, Aliesha Garrett,
Jennifer McKey, Patrick Havlik, Nikolai Rakhilin, Ergang Wang, Kun Xiang, Parker
Mathews, Lihua Wang, Cheryl Bock, Victor Ruthig, Yi Wang, Marcos Negrete, Chi Wut
Wong, Preetish K. L. Murthy, Shupei Zhang, Andrea R. Daniel, David G. Kirsch, Yubin
Kang, Blanche Capel, Aravind Asokan, Debra L. Silver, Rudolf Jaenisch, Xiling Shen
Materials/Methods, Supplementary Text, Tables, Figures, and/or References
- Download Supplement
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- Materials and Methods
- Figs. S1 to S8
- References
Images, Video, and Other Media
- Movie S1
- Embryonic heartbeat, indicating that the embryos were still alive.
- Movie S2
- Pups were delivered normally and survived birth.
- Movie S3
- The dam fed the pups normally.
- Movie S4
- Imaging a whole Wnt1-cre:tdTomato mouse embryo from E11.5 to E11.75 using a stereoscopic
microscope. - Movie S5
- Synaptic transmission between neurons, imaged using the calcium indicator GCaMP.
- Movie S6
- Diffusion of fluorescein into an embryo at E15.5 following injection into the dam
orbital vein. - Movie S7
- Movement of cells in an embryo at E14.5, 24 h after in utero electroporation to introduce pCAG-mCherry.
- Movie S8
- Cell migration in an embryo at E15.5, 24 h after in utero electroporation to introduce pCAG-eGFP.
- Movie S9
- Continuous imaging of a whole chimeric embryo, injected with eGFP-labeled mESCs in
blastocyst stage, for 12 h, from E11.5 to E12.0.
