I'm trying to electroporate NEB 5-alpha E. coli cells but have had issues with arcing despite following the recommendations from the protocol (using 25 μl of cells + 1 μl of DNA suspended water, pre-chilling 1 mm cuvettes, tapping out air bubbles, and setting the machine to 1.7 kV, ~145-250 Omega, and 25 μF).

Would greatly appreciate any help as last week 1 in 4 of the transformations worked but this week had no success.



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