Optimization of Electroporation and other Nonā€Viral Gene Delivery Strategies for T Cells












Biotechnology Progress



(
IF
2.334
)

Pub DateĀ :Ā 2020-08-17

, DOI:
10.1002/btpr.3066

Emily Harris; Jacob J. Elmer



CARā€T therapy is a particularly effective treatment for some types of cancer that uses retroviruses to deliver the gene for a chimeric antigen receptor (CAR) to a patient's T cells ex vivo. The CAR enables the T cells to bind and eradicate cells with a specific surface marker (e.g., CD19+ B cells) after they are transfused back into the patient. This treatment was proven to be particularly effective in treating nonā€Hodgkins lymphoma (NHL) and acute lymphoblastic leukemia (ALL), but the current CARā€T cell manufacturing process has a few significant drawbacks. For example, while lentiviral and gammaretroviral transduction are both relatively effective, the process of producing viral vectors is timeā€consuming and costly. Additionally, patients must undergo follow up appointments for several years to monitor them for any unanticipated side effects associated with the virus. Therefore, several studies have endeavored to find alternative nonā€viral gene delivery methods that are more inexpensive, precise, simple, and safe. This review focuses on the current state of the most promising nonā€viral gene delivery techniques, including electroporation and transfection with cationic polymers or lipids.


ę›“ę–°ę—„ęœŸļ¼š2020-08-18



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