been trying to electroporate plasmid dna into k562 cells but haven't been having much success. Our lab uses a biorad genepulser xcell that comes with preset settings for k562 cells in 2mm cuvettes but our lab uses 4mm cuvettes. I figured I could just double the voltage but that didn't work very well. Can anyone share a protocol or conditions they use to elecroporate plasmid dna into k562 cells with me? Thatd be great!



Source link