Highlights

Ce6 can be efficiently used for PDT with electroporation as one process (EP-PDT).

EP process significantly enhanced singlet oxygen generation by Ce6.

EP-PDT at lower temperatures induced better Ce6 uptake by Me45 melanoma cells.

EP-PDT at lower temperatures increased lipid peroxidation in Me45 melanoma cells.

Abstract

Melanoma is considered the most aggressive type of skin cancer, still without effective treatment. Thus, alternative therapeutic methods are still in demand, and electroporation-supported photodynamic therapy (EP-PDT) of cancer cells seems a promising approach. New developments in EP-PDT aim at enhanced tumor selectivity and biocompatibility by applying a second-generation photosensitizer, i.e., Chlorin e6 (Ce6). We have verified the improved photodynamic effect of Ce6 on skin cancer melanoma (Me45) cells and control (CHO-K1) cells. In this study, we applied 1 or 5 pulses of 10 ms duration and assessed the EP-PDT effect with a variety of tests, such as singlet oxygen scavenger (ABMDMA) photooxidation, oxidoreductive potential measurements, kinetic measurements with fluorescent microscopy, photosensitizer uptake studies, lipid peroxidation level, and actin fibers organization. The optimization of photosensitizer uptake as a function of temperature was also performed. Our results indicated efficient Ce6 delivery into Me45 cells and good photodynamic efficiency enhanced by the electroporation of cancer cells.

Keywords

Me45 cells

Chlorin e6

Electroporation

Photodynamic therapy

Singlet oxygen generation

Lipid peroxidation

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