High Efficiency Transformation of Bacillus brevis by Electroporation - PubAg




















Main content area

High Efficiency Transformation of Bacillus brevis by Electroporation

Author:
Okamoto, Akiko, Kosugi, Akihiko, Koizumi, Yukimichi, Yanagida, Fujiharu, Udaka, Shigezo
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.1 pp. 202-203
ISSN:
1347-6947
Subject:
Brevibacillus brevis, biotechnology, electroporation, plasmids, polyethylene glycol, single-stranded DNA
Abstract:
Various conditions in the Bacillus brevis transformation by electroporation were investigated to increase the efficiency. Competent cell volume and presence of polyethylene glycol 6000 and single stranded DNA on pulsing were critical for the efficiency. As a result, we established the improved method by which 10⁷–10⁸ transformants/μg plasmid DNA could be obtained.
Agid:
6917183




Source link