Genetic transformation of Pseudomonas oleovorans by electroporation
Abstract(#br)An electroporation procedure for the transformation of Pseudomonas oleovorans was developed using a model plasmid, pCN51. The optimal electrotransformation was achieved with cells harvested at 45 to 60 min of growth and concentrated to a cell density of 5 OD600nm, plasmid concentration of 6 μg per 100 μl of cell suspension, and a 0.1-cm gap-width cuvette. Electroporation was performed at the settings of 250 ω, 25μF and 2.5 kV. Transformation yields in the order of 103 colony-forming-unit per electroporation sample were obtained. This is a first report of the electroporation of the commercially valuable bacterium Ps. oleovorans. ? Rapid Science Ltd. 1998

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

电话:+86-010-53513060

网址:www.biovector.net



Source link