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Highlights

Highly efficient method for transfection of early chick embryos

Bilateral electroporation allows comparison of control and experimental conditions

Non-mosaic, highly reproducible results using various reagents

Summary

The chick embryo is a favored model for developmental studies owing to its accessibility and ease of manipulation. Ex ovo electroporation provides a highly efficient method for screening perturbation phenotypes using a variety of reagents, including CRISPR and morpholinos. Additionally, the chick system lends itself well to rapid medium-throughput enhancer screening. Constructs facilitating tissue-specific protein pull-down can also be transfected using this protocol. Furthermore, bilateral electroporation with control and experimental reagents provides a robust assay for accurately interpreting functional perturbations.

For complete details on the use and execution of this protocol, please refer to Williams et al. (2019).

Subject areas

High Throughput Screening

Model Organisms

© 2021 The Author(s).



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