Elektrotransfection

Gesundheit - iate.europa.eu

Hit-and-run programming of therapeutic cytoreagents using mrna nanocarriersCurrent widely used viral and electroporation methods for creating therapeutic cell-based products are complex and expensive...

Gesundheit - core.ac.uk - PDF: doaj.org

Xenogeneic human p53 dna vaccination by electroporation breaks immune tolerance to control murine tumors expressing mouse p53.... Mice immunized with xenogeneic full length hp53 DNA plasmid intramuscularly followed by electroporation were protected against challenge with murine colon cancer MC38 while those immunized with mp53 DNA were not...

Gesundheit - core.ac.uk - PDF: doaj.org

Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus rna using an improved electroporation method ... Here we report a useful system for the infection of tobacco protoplasts with a small amount of infectious RNAs, like in vitro transcripts from cDNA templates, involving an improved electroporation method...

Gesundheit - core.ac.uk -

Placental growth factor-2 gene transfer by electroporation restores diabetic sensory neuropathy in mice.... To examine the potential utility of PlGF-2 therapy for treating diabetic neuropathy, we performed intramuscular PlGF-2 gene transfer by electroporation, and examined its effects on sensory neuropathy in diabetic mice...

Journal of visualized experiments www.jove.com video article in utero and ex vivo electroporation for gene expression in mouse retinal ganglion... In chick, in ovo electroporation is used to manipulate gene expression in RGCs for examining retina and RGC development...

Electroporation for transfection and differentiation of dental pulp stem cells... Electroporation is a relatively safe and simple technique to deliver nucleic acids to the cell that acts by rendering cells transiently permeable using short periods of high voltage...

Gesundheit / Information und Informationsverarbeitung - core.ac.uk - PDF: www.liebertpub.com

Evaluation of safety of transdermal drug delivery using electroporation by in vitro and in vivo studiesIntroduction: Transdermal electroporation involves the application of high voltage electrical pulses for microsecond to millisecond duration to produce reversible increase in permeability of skin...

Effect of calcium electroporation in combination with metformin in vivo and correlation between viability and intracellular atp level after calcium electroporation in vitro.BACKGROUND:Calcium electroporation is a new experimental anti-cancer treatment where calcium is internalized into cells by application of short, high voltage pulses...

Gesundheit - core.ac.uk - PDF: doaj.org

Effect of calcium electroporation in combination with metformin in vivo and correlation between viability and intracellular atp level after calcium electroporation in vitro>BackgroundCalcium electroporation is a new experimental anti-cancer treatment where calcium is internalized into cells by application of short, high voltage pulses...

A dna vaccine for venezuelan equine encephalitis virus delivered by intramuscular electroporation elicits high levels of neutralizing antibodies in multiple animal models and provides protective immunity to mice and nonhuman primates▿We evaluated the immunogenicity and protective efficacy of a DNA vaccine expressing codon-optimized envelope glycoprotein genes of Venezuelan equine encephalitis virus (VEEV) when delivered by intramuscular electroporation...

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