Electroporation book: ISBN

This major reference work is a one-shot knowledge base on electroporation and the use of pulsed electric fields of high intensity and their use in biology, medicine, biotechnology, and food and environmental technologies. The Handbook offers a widespread and well-structured compilation of chapters ranging from the foundations to applications in industry and hospital. It is edited and written by most prominent researchers in the field. With regular updates and growing in its volume it is suitable for academic readers and researchers regardless of their disciplinary expertise, and will also be accessible to students and serious general readers. The authors of chapters have established scholarly credentials and come from a wide range of disciplines. This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment. An Editorial Board of distinguished scholars from across the world has selected and reviewed the various chapters to ensure the highest quality of this Handbook.ISBN:

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Electroporation is an electrical technique that involves the application of high-voltage electric pulses for very short duration (microsecond or millisecond) to enhance the skin permeability reversibly, for macromolecules. From: 50 V) to create transient pores in the stratum corneum, leading to a large increase in drug transport, delivery of macromolecules, a quick drug effect onset, and insignificant or only minor skin damage (). A combination of iontophoresis and electroporation could possibly further enhance drug transport, allowing rapid delivery of a bolus dose and precise control of drug delivery. The synergistic effects of iontophoresis (0.4 m A/cm). Blood glucose levels were significantly changed by the combined use of electroporation and iontophoresis, reflecting a plasma insulin concentration increase by approximately 23% in the 150-V group and 53% in the 300-V group 2 hours after administration against the initial level. Conversely, in some cases, lower combined effects than the effects with each individual treatment have been reported. studied the effects of iontophoresis and electroporation on the transdermal delivery of buprenorphine from solutions and hydrogels.

Lack of potent drug and gene delivery is one of the major problems of cancer chemotherapy and biotherapy. Different non-viral approaches have been proposed for drug and gene delivery such as physical and chemical methods. Physical delivery systems are one of the efficient non-viral methods including electroporation, micro-injection, gene gun, tattooing, laser and ultrasound [Bolhassani and Rafati, 2011]. Electroporation (EP) is the formation of aqueous pores in lipid bilayers by the application of a short (microseconds to milliseconds) high-voltage pulse to overcome the barrier of the cell membrane. This transient, permeabilized state can be used to load cells with a variety of different molecules including ions, drugs, dyes, tracers, antibodies, oligonucleotides, RNA and DNA [Faurie et al., 2005]. Electroporation has proven useful both is an effective method to increase cellular uptake of DNA and gene expression in tissue leading to marked improvement in immune responses. Electroporation represents a way of increasing the number of DNA-transfected cells and enhancing the magnitude of gene expression, while reducing intersubject variability and requiring less time to reach a maximal immune response compared to conventional intramuscular injection of the vaccine [Monie et al., 2010].
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