Effects of <it>in ovo </it>electroporation on endogenous gene expression: genome-wide analysis

By Chambers David, Gale Emily, Farley Emma K and Li Meng

Abstract

<p>Abstract</p> <p>Background</p> <p><it>In ovo </it>electroporation is a widely used technique to study gene function in developmental biology. Despite the widespread acceptance of this technique, no genome-wide analysis of the effects of <it>in ovo </it>electroporation, principally the current applied across the tissue and exogenous vector DNA introduced, on endogenous gene expression has been undertaken. Here, the effects of electric current and expression of a <it>GFP-</it>containing construct, via electroporation into the midbrain of Hamburger-Hamilton stage 10 chicken embryos, are analysed by microarray.</p> <p>Results</p> <p>Both current alone and in combination with exogenous DNA expression have a small but reproducible effect on endogenous gene expression, changing the expression of the genes represented on the array by less than 0.1% (current) and less than 0.5% (current + DNA), respectively. The subset of genes regulated by electric current and exogenous DNA span a disparate set of cellular functions. However, no genes involved in the regional identity were affected. In sharp contrast to this, electroporation of a known transcription factor, Dmrt5, caused a much greater change in gene expression.</p> <p>Conclusions</p> <p>These findings represent the first systematic genome-wide analysis of the effects of <it>in ovo </it>electroporation on gene expression during embryonic development. The analysis reveals that this process has minimal impact on the genetic basis of cell fate specification. Thus, the study demonstrates the validity of the <it>in ovo </it>electroporation technique to study gene function and expression during development. Furthermore, the data presented here can be used as a resource to refine the set of transcriptional responders in future <it>in ovo </it>electroporation studies of specific gene function.</p

Topics:
Neurology. Diseases of the nervous system, RC346-429

Publisher: BMC

Year: 2011

DOI identifier: 10.1186/1749-8104-6-17

OAI identifier:
oai:doaj.org/article:0984ece5c30947979c9c2df64944bc3c

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