Transient protein expression, is also known as transient gene expression. The proteins are expressed for a short duration. Majorly transient protein expressions are done with cultured animal cells. The technique is used in plant cells as well, however the transfer of proteins into these cells requires different methods than those with animal cells.
Stable transfer of cells and microorganisms is the key element for manufacturing of therapeutic proteins. Stably transfected mammalian cells in culture medium expand and further express their protein producing traits to their offspring’s. In transient protein expression new genes enter the host body carrying some protein making capability without becoming the component of organism’s genome. Various existing chemical and physical methods are used for achieving transient protein expression, these methods includes electroporation, gene guns and many others. These techniques impose limitations on per cell and volumetric productivity of transient protein production.
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Health care stakeholders are implementing payment reforms such as value-based payment models that help providers, payers, and patients achieve the best outcomes at the lowest cost. Technology-enabled patient engagement strategies are enabling increased financial independence for patients in their health care decisions, in addition to improving interactions with their health care systems. Governments are also moving the needle by adopting universal health coverage and introducing pricing controls on pharmaceuticals and medical technology devices. Population health management (PHM) is being used to identify people’s health care needs and offer services accordingly.
Big data and analytics will drive patient outcomes in a big way, and the coming years will witness a growing emphasis on healthcare service providers in the business of gathering and processing patient health data. Creating outcome-oriented financial models to maximize reimbursements in value-based healthcare will be a strategic shift from the traditional fees-for-services model. This implies that pharmaceutical companies will need to devise appropriate measures to optimize costs and improve the quality of patient care.
Mass technological transformation of the global healthcare industry has left key market participants struggling to traverse the complexities of medical and life sciences. Given the shifting dynamics of the global healthcare sector, pharma and biotech companies are taking measured efforts to improve patient outcomes in an effective manner, while keeping a careful watch on the bottom line
Transient protein expression is a speedy process when compared to stable integration. The stable transfection takes months and weeks while transient protein expression is accomplished in days, providing reliable and rapid access to small quantities of proteins for experimentation as well as characterization. Chemical based transient protein expression methods are used majorly due to low cost of transfection reagents and ease of use. Lipofection is one of the chemical based transient protein expression method which uses liposomes. The physical based transient protein expression method includes electroporation and uses high voltage electric shocks which induces DNA into the cells.
Factors Transient Protein Expression Market
The Transient Protein Expression market is driving with the increase in development of genetic engineering which has further opened many avenues and possibilities of expression and isolation of proteins in large scale. The various transient protein expression systems are yeast, bacteria, insect and mammalian systems. Considerable advances in technology and research have enabled transient protein expressions and isolation of recombinant proteins in large scale. However the success of transient protein expression depends on reproducibility, transfection efficiency which requires efficient researchers and reliable reagents to enhance the cell culture conditions. Moreover the market for transient protein expression is expected to increase due to its relatively inexpensive cost when compared to stable transfection also the timeline of expression is rapid. The transient protein expression is preferred more for small scale protein expression due to its rapid results.
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Moreover one of the major factor driving the improvement of the market includes increasing public-private funding for life science, research and presently commercially available very high-throughput devices for transient protein expressions. Healthcare facilities such as hospitals have changed their focus to a better value based system owing to improvement in healthcare. These factors stimulate the R&D and medical departments to overcome the deficiencies for better alternatives in Transient Protein Expressions.
Regional Market Outlook Transient Protein Expression Market
Regionally, Transient Protein Expression market is segmented into various regions. North America region is expected to dominate Transient Protein Expression market globally, followed by Europe, due to the rising adoption of technology in academic research centers and both research laboratories. The growing advancement in the technology with use of cost effective devices replacing the traditional systems with the newest technologies is increasing the market for Transient Protein Expression devices in the region. The areas such as cancer research, plants and animal genomics, clinical research, and diagnostics, will benefit due to Transient Protein Expressions
Some of the major players in the Transient Protein Expression Market are Merck KGaA, Lonza, Thermo Fisher Scientific, PhyNexus, Inc. Molecular Devices, LLC, Sartorius AG, Promega Corporation among others. Moreover, the manufacturers of Transient Protein Expressions are mainly focusing on strengthening and increasing the core competencies of their product portfolio.
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