Elsevier

Available online 2 July 2020, 107592

Bioelectrochemistry

Highlights

Calcium electroporation is effective on the Rhabdomyosarcoma cell line.

CaEP therapy induces activation of apoptosis and caspase 3/7.

CaEP is not cytotoxic for normal C2C12 muscle cells.

Abstract

Calcium electroporation (CaEP) has been previously reported as an effective method of rhabdomyosarcoma cells reduction. CaEP causes temporary cell membrane permeabilization with simultaneous calcium ions influx. A rapid influx of calcium ions leads to mitochondrial overload by Ca2+, loss of mitochondrial membrane potential causing cytochrome c release, caspase cascade activation and, as a consequence, cell death. This study was conducted on two cell lines: normal muscle cells (C2C12) and rhabdomyosarcoma cells (RD), which showed different cellular responses to CaEP. Our study defined apoptosis as the main cell death type occurring after CaEP in RD cells. Increased activity of caspase 3/7, Parp-1 and cleaved Parp-1 were proven in the case of RD cells. RD cells compartment rearrangement was observed in the time-lapse by holotomographic microscopy (HTM). C2C12 cells were less sensitive to electroporation and increased Ca2+ concentration, and viability was maintained at the level of control cells, only slight changes in pro-apoptotic factors were observed. The results reveal CaEP as a promising therapeutic approach in cancers which develop from muscle tissue.

Keywords

rhabdomyosarcoma

muscle cells

calcium ions

electroporation

caspase 3

apoptosis

Abbreviations

CaEP

calcium electroporation

CLSM

Confocal laser scanning microscopy

PTP

permeability transition pores

PARP 1

poly (ADP-ribose) polymerase-1

MOMP

mitochondrial outer membrane permeabilization

ROS

reactive oxygen species

ATP

Adenosine triphosphate

NAD(P)

Nicotinamide adenine dinucleotide phosphate

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© 2020 Published by Elsevier B.V.



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