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Description

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ElectroporationKitforHL-60Cells (PlasmacytomaCells,CCL-240)

 •ElectroporationKitinclude:electroporationbuffer+ buffer-specificoptimizedelectroporationprotocols
•Pre-optimizedelectroporationprotocolsareprovidedfordeliveryofplasmids(DNA), siRNA,mRNA,andproteins
•Proprietaryelectroporationbuffer formulationmimicscellularcytoplasmcomposition(superiorelectroporeresealing)s
•CertifiedRNAse-andDNAse-free electroporationbuffer
• Effective electroporationdeliveryandhighcellviABIlity providesreproducIBLeelectroporationresults
•Highcellviabilityandsuperiorcellrecoveryrates
• Electroporationprotocolsare providedwithwiththepurchaseoftheElectroporation Kit
•DevelopedandmanufacturedbyAltogenBiosystems

ElectroporationBuffer:
4mLofelectroporationbuffer issufficientfor20 reactions usingstandard0.2cmelecrtoporationcuvette
30mLofelectroporationbuffer issufficientfor150 reactions usingstandard0.2cmelecrtoporationcuvette

ElectroporationEfficiency:
Electroporationkitprovides 65-80%delivery efficiencyofsiRNA.Electroporation efficiencywasdeterminedbyqRT-PCR.

Electroporation ProtocolandMSDS:
ElectroporationprotocolsareprovidedwiththepurchaseoftheElectroporationKit
DownloadMSDS:[PDF]

HL-60CellLine:
ThetumOrigenicHL-60celllinewasestablishedusingtheperipheralbloodtissuefroma36-year-oldfemale patientwhosufferedfromacutepromyelocyticleukemia(APL).Thiscelllineexhibitsmyeloblasticcellmorphologyandapromyeloblastcelltype.Thesecellspositivelyexpressthemyconcogene,andwasderivedbyS.J.Collins,etall.HL-60havebeenknowntospontaneouslydifferentiate,thiscanevenbestimulatedandcontrolled,toanextent,byavarietyofcompounds.Thiscelllineisusefulasatransfectionhostinscientificresearch.

Data:
HL60 Electroporation Kit

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

  • 2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
  • Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
  • NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
  • CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]

RequestaQuotefor
HL-60ElectroporationKit

AltogenBiosystems:

AltogenBiosystemsprovidespreoptimizedtransfectionproductsforlifescienceresearchapplications.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,RNA,andanyothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

  • 4ml(Catalog#1751)
  • 30ml(Catalog#1752)



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